Molecular Biology And Biotechnology Principles

Recombinant DNA Technology , Polymerase Chain Reaction ,Electrophoresis , Chromatography, Blotting technique and ELISA

What you'll learn
Recombinant DNA Technology or Cloning
Polymerase Chain Reaction
Hybridisation and Blotting Techniques
Principles of Chromatography and Electrophoresis
Principles of electrophoresis
Requirements
Basic concept of biology and should know basic things about Biotechnology
Description
In this course students will learn about molecular biology and biotechnology principles.1. Unit 1Topics covered in : Lecture 1 , 2 and 3Recombinant DNA technology Tools used in Recombinant DNA technologyIsolation of vector gene Isolation of desired geneMethods to transfer foreign gene into bacterial cellDiscuss about Restriction enzymesAnd about ligases Vectors like cosmid, plasmid Production of insulin gene Applications of recombinant DNA technology2. Unit 2Topics covered in Lecture 4 Molecular biology TechniquesSouthern blotting technique used for DNA samplesNorthern blotting used for RNA samples 3. Unit 3Topics covered in Lecture 5Hybridisation technique Polymerase chain reaction used to amplify the DNA in the sample Radio immuno Assay - Antigen and antibody specific Electrophoresis zone electrophoresis Gel electrophoresis Polyacrylamide gel electrophoresis.Paper electrophoresis.Principle of electrophoresis.Used to separate the DNA depending on the size and charge on the molecule.Gel electrophoresis - is used to separate DNA or RNA.SDS page used to separate proteins and amino acids4. Unit 4Topics covered in Lecture 6 and 7 chromatographycolumn chromatography Affinity chromatography.- based on affinity towards ligand and protein .Ion exchange chromatography.Cat-ionic exchanger and anionic exchangerSeparation is based on ions present in the protein sample Gas liquid chromatography. Gas acts as a mobile phase and liquid acts as a stationary phase .gases like inert gases are used and liquid of high boiling point is used in gas liquid chromatographyPaper chromatography . Paper is used and reagent are used to identify the compound present in the sample principle of chromatography separation of molecules based on size, charge and molecular weight Separation of macro molecules based on antigen and antibody interactionEnzyme and ligand interaction as there is a specific interaction between the antigen and antibody interaction Summary of the courseAdd-onCourse would be added/enhanced based on Q&A form students in to form of additional information

Overview

Section 1: Introduction

Lecture 1 Introduction

Section 2: Unit 1

Lecture 2 Recombinant DNA technology

Lecture 3 Lecture 2

Lecture 4 Lecture 3

Section 3: Section : Unit 2

Lecture 5 Lecture 4

Section 4: Section : Unit 3

Lecture 6 Lecture 5

Section 5: Unit 4

Lecture 7 Lecture 6

Lecture 8 Lecture 7

Section 6: Summary of the course

Lecture 9 Summary of the course

Students who are in the field of Molecular Biology ,Biotechnology and students interested in Research Field

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